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Purification of labeled antibody by minicolumn gel centrifugation

✍ Scribed by Alan J. Parkinson; E.N. Scott; H.G. Muchmore

Publisher: Elsevier Science
Year: 1981
Tongue: English
Weight: 305 KB
Volume: 118
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(81)90602-3

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✦ Synopsis

Radiolabeled anti-human immunoglobulin G was purified by the elution from a minicolumn of Protein A-Sepharose by gel centrifugation. As much as 93.7% of the Protein A-bound '*'Ilabeled immunoglobulin G was recovered within the first three 200~~1 fractions. Removal of the eluting agent was achieved by centrifuging the peak fractions through a minicolumn of hydrated Sephadex G-50.

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