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Purification of eukaryotic ribosomes by isopycnic centrifugation in sucrose

✍ Scribed by P. Nieuwenhuysen; H. Slegers

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 506 KB
Volume: 89
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90376-7

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✦ Synopsis

A simple and inexpensive procedure for the isolation and purification of ribosomes from eukaryotes is described. The method avoids pelleting of ribosomes at high centrifugal forces and involves isopycnic centrifugation of the postmitochrondrial supernatant in sucrose, precipitation of ribosomes with 10% polyethylene glycol, and zonal sucrose gradient centrifugation. The ribosomes obtained in this way are very pure and thus especially suited for the measurements of physical properties. The isopycnic centrifugation can also be used for the purification of other macromolecules and is only limited by a maximum density of sucrose of 1.40 g/cm" obtained at the bottom of the centrifugation tubes.

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