✦ LIBER ✦
Repair replication in human cells studied by sodium lodide isopycnic centrifugation of DNA in a fixed-angle rotor
✍ Scribed by P.H.M. Lohman; M.L. Sluyter; I.A.A. Matthijs; W.J. Kleijer
- Publisher
- Elsevier Science
- Year
- 1973
- Tongue
- English
- Weight
- 568 KB
- Volume
- 54
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A sensitive and quantitative method is described for direct measurement of repair replication in uv-irradiated human cells in tissue culture. The method is based on isopycnic centrifugation of repaired DNA in sodium iodide gradients in fixed angle rotors and determination of the amount, of DNA by the fluorescence of a DNA ethidium bromide complex.