✦ LIBER ✦

Altered DNA sedimentation by the presence of erythrocytes in alkaline sucrose gradient centrifugation

✍ Scribed by Tetsuo Suda; Mitsuhiro Omine; Tadashi Maekawa

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 349 KB
Volume: 89
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90360-3

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✦ Synopsis

The presence of a relatively small number of red cells was found to affect DNA sedimentation profile of normal lymphocytes and acute leukemia cells, as observed by the alkaline sucrose gradient centrifugation technique coupled with the fluorometric measurement of DNA. Significant alteration was observed at a nucleated celherythrocyte ratio of 20/l to 0.211, resulting in retardation of the Sill value and the entire sedimentation profile. This effect seemed to be rather specific to erythrocyte lysate, since corresponding amounts of erythrocyte ghost, IgM, bovine serum albumin, and an increased number of nucleated cells did not influence the profile to an appreciable degree.

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