Rapid purification of DNA from agarose gels by centrifugation through a disposable plastic column

✍ Scribed by Bert Vogelstein

• Publisher: Elsevier Science
• Year: 1987
• Tongue: English
• Weight: 637 KB
• Volume: 160
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(87)90620-8

✦ Synopsis

A simple and rapid method for purifying DNA from agarose gels is described. Agarose slices containing DNA are placed in a disposable plastic column and the DNA is separated from the agarose by centrifugation in a microfuge. Recoveries averaging 25% are obtained for DNA of 14 kb or less. The recovered DNA can be labeled to high specific activity, cleaved with restriction endonucleases, and ligated efficiently using standard cloning vectors. o 1987 Academic RUSS, he.