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Purification of heart hexokinase by dye-ligand chromatography

✍ Scribed by Edward E. Farmer; John S. Easterby

Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 398 KB
Volume: 123
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(82)90460-2

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✦ Synopsis

Heart hexokinase (EC 2.7.1.1) has been purified by a procedure that uses affinity elution from a column of red triazine dye, H-IBN, immobilized to Sepharose 6B. Homogeneous protein (9 mg) is obtained from 500 g heart in 40% yield. The mechanism of affinity elution from the dye is discussed.

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