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Purification of H-2a heavy chain and β2-Microglobulin by reverse-phase high-performance liquid chromatography

✍ Scribed by Vernon L. Alvarez; Carolyn A. Roitsch; Ole Henriksen

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
398 KB
Volume
115
Category
Article
ISSN
0003-2697

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✦ Synopsis

Partially

purified, papain-solubilized H-2" histocompatibility antigens from A/J mouse liver have been purified by reverse-phase high-performance liquid chromatography using RP-18 columns. &-Microglobulin and H-2" heavy chain were separated from each other, without reduction or carboxymethylation, in less than 30 min by elution with a linear gradient of ethanol in I2 mM HCI. As much as 5 mg of protein has been loaded at a time. The recovery of the proteins from the RP-I8 columns was estimated to be approximately 35% for the H-2a heavy chain and 80% for &microglobulin.

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