A simple and rapid purification of commercial trypsin and chymotrypsin by reverse-phase high-performance liquid chromatography
โ Scribed by Koiti Titani; Tatsuru Sasagawa; Katheryn Resing; Kenneth A. Walsh
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 485 KB
- Volume
- 123
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
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The enzymatic syntheses of ATP analogs, such as tubercidin 5 '-triphosphate, formycin A 5 '-triphosphate, and etheno-ATP, from their respective mono- and diphosphate are described. The reaction products were purified by reverse-phase HPLC using a C-18 matrix and a volatile mobile phase at \(\mathrm{
## Partially purified, papain-solubilized H-2" histocompatibility antigens from A/J mouse liver have been purified by reverse-phase high-performance liquid chromatography using RP-18 columns. &-Microglobulin and H-2" heavy chain were separated from each other, without reduction or carboxymethylati