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Purification of Glucose Oxidase and β‐Galactosidase by Partitioning in a PEG‐Salt Aqueous Two‐Phase System in the Presence of PEG‐Derivatives

✍ Scribed by Dhoot, Shrikant B.; Dalal, Juned M.; Gaikar, Vilas G.


Book ID
126527799
Publisher
Taylor and Francis Group
Year
2007
Tongue
English
Weight
261 KB
Volume
42
Category
Article
ISSN
0149-6395

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PEG has been activated using epoxy-oxirane, epichlorohydrin and periodate based reactions. The coupling to activated PEG of several protein ligands of different sizes was investigated. Glutathione, trypsin inhibitor, Protein A and anti-BSA have been bound to PEG and used to increase the selectivity