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Purification of dibenzothiophene monooxygenase from a recombinantEscherichia coli

โœ Scribed by Nidhi Gupta; D. K. Adhikari; T. Stobdan; P. K. Roychoudhury; J. K. Deb

Publisher
Springer Netherlands
Year
2007
Tongue
English
Weight
229 KB
Volume
29
Category
Article
ISSN
0141-5492

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A single-step procedure is small scale (annrox. 5mg) described for purifying a-lytic protease and its mutants on a from cell-free supematants of recombinant E. coli. Although free of non-enzyme pro&t, these preparations often contain fragments that arise from enzymatic self-digestion. Two strong cat