The usefulness of size-exclusion high-performance liquid chromatography (HPLC) for the molecular weight determination of allergen extracts as a method of process control was investigated by measuring seven different batches of timothy pollen extracts. Preparative HPLC was used for the isolation of t
Purification of allergens by high-performance liquid chromatography : IV. Purification of the allergen of olive pollen (Olea europea)
β Scribed by Nazario Rubio; Aurora Brieva; Bernardino Alcazar
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 333 KB
- Volume
- 403
- Category
- Article
- ISSN
- 1873-3778
No coin nor oath required. For personal study only.
β¦ Synopsis
The olive (Olea europea) is an important cause of pollen allergy in Mediterranean countries. Around 50% of pollinic patients in Spain give a positive reaction to olive extracts in the skin prick test'. The pollen grain of Oiea europea is around 20 pm in diameter and it reaches concentrations of 50 grains/m3 in the pollinating season (May-June)*.
No attempts to purify olive allergens have been performed to our knowledge. Here we report the purification of the main allergen of Olea europea using distilled water as the eluent in Protein Pak 125 (Waters Assoc.) columns.
EXPERIMENTAL
π SIMILAR VOLUMES
A simple two-step procedure for obtaining highly purifed human tibrinopeptides is described. This technique utilizes batch adsorption to a Clll silica-coated matrix packed in a disposable cartridge. This step serves both to concentrate and desalt fibrinopeptides released into the supernatant during
Adenovirus (Type 2)-infected HeLa cells were sonicated and treated with 1,1,2-trichlorotrifluoroethane. The water-soluble extract was ultracentrifuged and the supernatant, containing the dissociated proteins, was subjected to anion-exchange high-performance liquid chromatography on a Mono Q column i
## Abstract A sample of [^3^H]β8βmethoxypsoralen was purified by high performance liquid chromatography on silica gel.