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Purification of allergens by high-performance liquid chromatography : IV. Purification of the allergen of olive pollen (Olea europea)

✍ Scribed by Nazario Rubio; Aurora Brieva; Bernardino Alcazar


Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
333 KB
Volume
403
Category
Article
ISSN
1873-3778

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✦ Synopsis


The olive (Olea europea) is an important cause of pollen allergy in Mediterranean countries. Around 50% of pollinic patients in Spain give a positive reaction to olive extracts in the skin prick test'. The pollen grain of Oiea europea is around 20 pm in diameter and it reaches concentrations of 50 grains/m3 in the pollinating season (May-June)*.

No attempts to purify olive allergens have been performed to our knowledge. Here we report the purification of the main allergen of Olea europea using distilled water as the eluent in Protein Pak 125 (Waters Assoc.) columns.

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