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Purification of adenovirus hexon protein by high-performance liquid chromatography

✍ Scribed by Matti Waris; Pekka Halonen

Publisher: Elsevier Science
Year: 1987
Tongue: English
Weight: 459 KB
Volume: 397
Category: Article
ISSN: 1873-3778
DOI: 10.1016/s0021-9673(01)85015-9

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✦ Synopsis

Adenovirus (Type 2)-infected HeLa cells were sonicated and treated with 1,1,2-trichlorotrifluoroethane. The water-soluble extract was ultracentrifuged and the supernatant, containing the dissociated proteins, was subjected to anion-exchange high-performance liquid chromatography on a Mono Q column in 50 mM bis-Tris-HCl (pH 6.5). Elution with a linear salt gradient resulted in a major peak, containing the hexon protein.

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