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Molecular weight determination of allergen extracts and isolation of allergenic molecules by high-performance liquid chromatography

✍ Scribed by Rüdiger Wahl; Dietmar Meineke; Hans Jörg Maasch

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
710 KB
Volume
397
Category
Article
ISSN
1873-3778

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✦ Synopsis

The usefulness of size-exclusion high-performance liquid chromatography (HPLC) for the molecular weight determination of allergen extracts as a method of process control was investigated by measuring seven different batches of timothy pollen extracts. Preparative HPLC was used for the isolation of the major allergen Cat 1, from a crude cat hair and skin scraping (CHSS) extract. The chromatograms of the seven batches of the timothy pollen extracts looked very similar, suggesting the same molecular weight distribution in each extract. The major allergen, Cat 1, could be isolated from the crude CHSS extract by preparative HPLC in acceptable purity, determined by crossed radioimmunoelectrophoresis.

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