Purification of a lipase from the hepatopancreas of oil sardine (Sardinella longiceps linnaeus) and its characteristics and properties

✍ Scribed by M. K. Mukundan; K. Gopakumar; M. R. Nair

• Book ID: 102923448
• Publisher: John Wiley and Sons
• Year: 1985
• Tongue: English
• Weight: 568 KB
• Volume: 36
• Category: Article
• ISSN: 0022-5142
• DOI: 10.1002/jsfa.2740360310

✦ Synopsis

Lipase has been purified from the hepatopancreas of oil sardine (Sardinellu longiceps) by defatting, water extraction, ammonium sulphate fractionation and chromatography on D E A E Sephadex and Sephadex G-100. The preparation was homogeneous on polyacrylamide disc gel electrophoresis and on gel filtration through Sephacryl S-200. The enzyme showed a molecular weight of 54000-57000 with 6.1% of carbohydrate. The p H and temperature optima of purified sardine lipase were 8 and 37°C respectively. Sardine lipase remained stable up to 45°C (15 min) and in the p H range 5 to 9.5. The K, values obtained for the substrates tributyrin and triacetin were 4X lo-' and 30X lo-', respectively. The effect of halogens and various metal ions on sardine lipase activity, substrate specificity, amino acid and carbohydrate composition are also reported.