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Purification, Crystallization, and Preliminary X-ray Diffraction Analysis of the Tricorn Protease Hexamer from Thermoplasma acidophilum

✍ Scribed by Jürgen Bosch; Tomohiro Tamura; Gleb Bourenkov; Wolfgang Baumeister; Lars-Oliver Essen


Book ID
115634206
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
250 KB
Volume
134
Category
Article
ISSN
1047-8477

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The recombinant homodimeric hemoglobin from the strictly aerobe gram-negative bacterium Vitreoscilla stercoraria has been expressed in Escherichia coli, purified to homogeneity, and crystallized by vapor diffusion techniques, using ammonium sulfate as precipitant. The crystals belong to the monoclin