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Purification and some properties of guanidinoacetate amidinohydrolase produced by Corynebacterium sp

โœ Scribed by Shirokane, Yoshio; Nakajima, Moto-o


Book ID
123012580
Publisher
Elsevier Science
Year
1986
Weight
548 KB
Volume
64
Category
Article
ISSN
0385-6380

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Formate: NAD+ oxidoreductase (EC 1.2.1.2) with high affinity to formate was purified from a facultative formate-utilizing Agrobacterium sp. MIL 1052. From a cell extract, the enzyme was purified to homogeneity by ammonium sulfate fractionation, DEAE-Sephadex A-50 column chromatography, Sephadex (3-2