Purification and some properties of the extracellular chitinase produced by Trichoderma harzianum
β Scribed by Cirano J. Ulhoa; John F. Peberdy
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 537 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0141-0229
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BACKGROUND: Purification and enzymatic properties of a chitosanase from Bacillus subtilis RKY3 have been investigated to produce a chitooligosaccharide. The enzyme reported was extracellular and constitutive, which was purified by two sequential steps including ammonium sulfate precipitation and ion
## Abstract **BACKGROUND:** Chitoβoligosaccharide (COS) is generally known to possess many specific biological functions, especially antibacterial activity, depending on its size. To prepare a specific size range of COS, however, has proved difficult. The aim of this study was to establish a method