Purification and properties of NAD+-dependent maltose dehydrogenase produced by alkalophilic Corynebacterium sp. No. 93-1

Purification and properties of NAD+-depe

Purification and properties of NAD+-dependent formate dehydrogenase produced by Agrobacterium sp.

✍ Dr. Mitsugi Iida; Shin'Ichi Ohtsuki; Shigeru Mineki 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 550 KB

Formate: NAD+ oxidoreductase (EC 1.2.1.2) with high affinity to formate was purified from a facultative formate-utilizing Agrobacterium sp. MIL 1052. From a cell extract, the enzyme was purified to homogeneity by ammonium sulfate fractionation, DEAE-Sephadex A-50 column chromatography, Sephadex (3-2