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Purification and properties of reconstitutively active nicotinamide nucleotide transhydrogenase of Escherichia coli

✍ Scribed by David M. CLARKE; Philip D. BRAGG


Book ID
115123081
Publisher
John Wiley and Sons
Year
1985
Tongue
English
Weight
979 KB
Volume
149
Category
Article
ISSN
1432-1327

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The conditions of extraction of nicotinamide nucleotides from Escherichia coli were systematically investigated. The optimum pH for NAD+ and NADP+ appeared to be around 1.5 while that for NADH and NADPH was around 12.5. Seven minutes at 60°C was chosen for the extraction of all four nucleotides. Add