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Purification and molecular properties of reconstitutively active nicotinamide nucleotide transhydrogenase from beef heart mitochondria

✍ Scribed by Bo Höjeberg; Jan Rydström


Book ID
118311942
Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
458 KB
Volume
78
Category
Article
ISSN
0006-291X

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Soluble, oligomycin-insensitive ATPase released from beef heart mitchondria by chloroform extraction can be further purified by Sepharose 6B gel filtration. This purification increases enzyme activity 4--5 times (100-130U/mg). According to specific activity, high purity and ability to reconstitute o