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Purification and characterization of α-glucosidase fromAspergillus carbonarious

✍ Scribed by Dey-Chyi Sheu; Kow-Jen Duan; Chi-Tsai Lin


Publisher
Springer-Verlag
Year
1994
Tongue
English
Weight
364 KB
Volume
8
Category
Article
ISSN
0951-208X

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✍ C. Dissous; J.F. Ansart; A. Cheron; J. Krembel 📂 Article 📅 1981 🏛 Elsevier Science 🌐 English ⚖ 571 KB

## Lysosomal u-glucosidase (EC 3.2.1.20) was purified from a lysosome-enriched fraction of rat liver using an improved procedure. A purification factor of 2900-fold was reached, with a yield of 35%. Polyacrylamide gel electrophoresis of the purified enzyme in nondenaturing conditions, or in the p