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Purification and characterization of the DNA binding domain of Saccharomyces cerevisiae meiosis-specific transcription factor Ndt80

✍ Scribed by Richelle Sopko; David T Stuart


Book ID
116827733
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
363 KB
Volume
33
Category
Article
ISSN
1046-5928

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A 124-residue N-terminal fragment corresponding to the DNA-binding domain of the Saccharomyces cerevisae cell-cycle transcription factor MBP-1 has been expressed with a hexahistidine affinity tag in E. coli and purified to apparent homogeneity. Crystals have been grown using PEG 3350 as precipitant