Expression, purification, and crystalliz
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Ian A. Taylor; Stephen J. Smerdon
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Article
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1997
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John Wiley and Sons
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English
β 31 KB
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A 124-residue N-terminal fragment corresponding to the DNA-binding domain of the Saccharomyces cerevisae cell-cycle transcription factor MBP-1 has been expressed with a hexahistidine affinity tag in E. coli and purified to apparent homogeneity. Crystals have been grown using PEG 3350 as precipitant