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Expression and Purification of the DNA-Binding Domain of the Human Transcription Factor E2F1

✍ Scribed by Wangyong Zeng; Xuecheng Zhang; Xiaoming Tu; Shouhong Guang; Yazhong Xiao; Yunyu Shi


Book ID
115645763
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
109 KB
Volume
21
Category
Article
ISSN
1046-5928

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✍ Ian A. Taylor; Stephen J. Smerdon πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 31 KB πŸ‘ 2 views

A 124-residue N-terminal fragment corresponding to the DNA-binding domain of the Saccharomyces cerevisae cell-cycle transcription factor MBP-1 has been expressed with a hexahistidine affinity tag in E. coli and purified to apparent homogeneity. Crystals have been grown using PEG 3350 as precipitant