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Purification and characterization of plasma membranes from Physarum polycephalum amoebae

✍ Scribed by Anne Barden; Gérald Lemieux; Dominick Pallotta


Book ID
115726216
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
697 KB
Volume
730
Category
Article
ISSN
0005-2736

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The myxomycete Physarum polyeephalum has been reported to produce an extracellular ribonuclease. A quick procedure is described for the isolation and purification of the ribonuclease from the culture supernatant. It involves an initial concentration of the culture supernatant by slow freezing, follo