The myxomycete Physarum polyeephalum has been reported to produce an extracellular ribonuclease. A quick procedure is described for the isolation and purification of the ribonuclease from the culture supernatant. It involves an initial concentration of the culture supernatant by slow freezing, follo
โฆ LIBER โฆ
Purification and some properties of hemagglutinin from the Myxomycete,Physarum polycephalum
โ Scribed by M. Yokota; K. Nitta
- Publisher
- Springer
- Year
- 1996
- Tongue
- English
- Weight
- 535 KB
- Volume
- 52
- Category
- Article
- ISSN
- 1420-682X
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Using flow cytometry, spherulating nuclei of Physarum isolated at the beginning of spherule wall formation were found to exhibit a DNA content corresponding to the G phase of the cell cycle, although 8% lower. Before the first mitosis after spherule germination, a very slight incorporation of H thym