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Isolation and purification of the extracellular ribonuclease from Physarum polycephalum

✍ Scribed by Farr, D. R. ;Amster, H. ;Horisberger, M.

Publisher
Springer-Verlag
Year
1972
Weight
232 KB
Volume
85
Category
Article
ISSN
0003-9276

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✦ Synopsis

The myxomycete Physarum polyeephalum has been reported to produce an extracellular ribonuclease. A quick procedure is described for the isolation and purification of the ribonuclease from the culture supernatant. It involves an initial concentration of the culture supernatant by slow freezing, followed by ammonium sulfate precipitation. The enzyme was adsorbed onto DEAE-cellulose and eluted with a sodium chloride gradient.

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A method for separation of pigments from
A method for separation of pigments from plasmodia of the true slime molds, Physarum polycephalum and Physarum nudum
✍ Andrzej Majcherczyk; Leokadia Rakoczy; Aloys HΓΌttermann πŸ“‚ Article πŸ“… 1987 πŸ› Elsevier Science 🌐 English βš– 455 KB

Plasmodia of the true slime molds Physarum polycephalum and Physarum nudum were grown on agar semidefined medium (J. W. Daniel and H. Baldwin (1964) in Methods in Cell Physiology (Precsott, D. M., Ed.), Vol. 1, pp. 9-44, Academic Press, New York.) and pigments were extracted from 8-day-old plasmodia