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Purification and characterization of phosphofructokinase in bovine parotid gland

✍ Scribed by Fukushima Eiichi; Sugiya Hiroshi

Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
804 KB
Volume
24
Category
Article
ISSN
0020-711X

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Phosphofructokinase from Kluyveromyces lactis was purified by 180-fold enrichment, elaborating the following steps: cell disruption, polyethylene glycol precipitation, affinity chromatography, size exclusion chromatography on Sepharose 6B and on Bio-Sil SEC 400 and ion exchange chromatography. The h