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Purification and characterization of an ADP-dependent phosphofructokinase fromThermococcus zilligii

✍ Scribed by R. S. Ronimus; Jurre Koning; H. W. Morgan


Publisher
Springer
Year
1999
Tongue
English
Weight
124 KB
Volume
3
Category
Article
ISSN
1431-0651

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Glutathione reductase has been purified to at least 98% homogeneity from calf liver. An essential part in the procedure involves affinity chromatography on 2',5'-ADP-Sepharose 4B to which the enzyme remains bound in the presence of 0.4 M phosphate. This step separates glutathione reductase from the