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Purification and characterization of pyrophosphate- and ATP-dependent phosphofructokinases from banana fruit

✍ Scribed by William L. Turner; William C. Plaxton

Publisher
Springer-Verlag
Year
2003
Tongue
English
Weight
270 KB
Volume
217
Category
Article
ISSN
0032-0935

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Phosphofructokinase from Kluyveromyces lactis was purified by 180-fold enrichment, elaborating the following steps: cell disruption, polyethylene glycol precipitation, affinity chromatography, size exclusion chromatography on Sepharose 6B and on Bio-Sil SEC 400 and ion exchange chromatography. The h