Purification and Characterization of Alkaline Proteinase from AlkalophilicBacillussp.
โ Scribed by A. Muderriszade; N. Y. Ensari; S. Aguloglu; B. Otludil
- Book ID
- 110312088
- Publisher
- SP MAIK Nauka/Interperiodica
- Year
- 2001
- Tongue
- English
- Weight
- 32 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0003-6838
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A newly isolated Bacillus species, which grew optimally at 30ยฐC and pH 10, produced a carboxymethylcellulase in a medium containing 10 g CM-cellulose/l. The enzyme, when partially purified by gel filtration, had a mass of about 29 kDa as determined by both SDS-PAGE and gel filtration chromatography.
A /3-cyclodextrin glucosyltransferase was purified from alkalophilic Bacilhs sp. No. 562 over 64-fold with a yield of 32%. Its molecular size was estimated to be 170 kDa by gel &ration and 82 kDa by SDS-PAGE, with a pI of 7.2. The enzyme showed optimum activity at 65ยฐC and pH 7.0. It was stable from