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Partial purification and some properties of an alkaline cellulase from an alkalophilicBacillussp.

โœ Scribed by H. Khyami-Horani

Publisher
Springer
Year
1996
Tongue
English
Weight
532 KB
Volume
12
Category
Article
ISSN
1573-0972

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โœฆ Synopsis

A newly isolated Bacillus species, which grew optimally at 30ยฐC and pH 10, produced a carboxymethylcellulase in a medium containing 10 g CM-cellulose/l. The enzyme, when partially purified by gel filtration, had a mass of about 29 kDa as determined by both SDS-PAGE and gel filtration chromatography. It was optimally active at pH 9.5 and 40ยฐC, and was stable from pH 7 to 11 at 4ยฐC for 24 h. The enzyme was stimulated by Ca(2+) (1mM) but was completely inhibited by Hg(2+) (1MM). Neither EDTA nor EGTA (10MM) affected the activity.

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