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Purification and characterization of a β-cyclodextrin glucosyltransferase from an alkalophilicBacillussp.

✍ Scribed by Tsong-Rong Yan; Tsu-Tai Lin

Publisher
Springer-Verlag
Year
1995
Tongue
English
Weight
365 KB
Volume
9
Category
Article
ISSN
0951-208X

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✦ Synopsis

A /3-cyclodextrin glucosyltransferase was purified from alkalophilic Bacilhs sp. No. 562 over 64-fold with a yield of 32%. Its molecular size was estimated to be 170 kDa by gel &ration and 82 kDa by SDS-PAGE, with a pI of 7.2. The enzyme showed optimum activity at 65°C and pH 7.0. It was stable from 0 to70 "C and from pH 7.0 to 11.0. The enzyme was specifically inhibited by Fe2+ and Fe3+.

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