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Purification and characterization of a novel neutral β-glucanase and an alkaline β-glucanase from an alkaliphilicBacillusisolate

✍ Scribed by Sen-Lin Liu; Wei-Zhao Chen; Yong Wang; Gang Liu; Shao-Wen Yu; Miao Xing

Publisher
Springer
Year
2007
Tongue
English
Weight
350 KB
Volume
24
Category
Article
ISSN
1573-0972

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Purification and characterization of an
Purification and characterization of an extracellular β-glucanase from Bacillus IMET B 376
✍ Dr. sc. R. Borriss 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 613 KB

## Abstract β‐1.3‐1.4‐glucanase (E.C.3.2.1.73) was obtained in highly purified form from the culture fluid of __Bacillus__ IMET B 376 by precipitation with ammonium sulfate, adsorption on CM‐cellulose and then affinity chromatography on lichenan‐Sepharose 4B. The purified enzyme was active on liche