Purification and characterization of a β-d-mannosidase from the marine anaspidean Aplysia fasciata
✍ Scribed by Giuseppina Andreotti; Assunta Giordano; Annabella Tramice; Ernesto Mollo; Antonio Trincone
- Book ID
- 118471378
- Publisher
- Elsevier Science
- Year
- 2005
- Tongue
- English
- Weight
- 221 KB
- Volume
- 119
- Category
- Article
- ISSN
- 0168-1656
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A 1,2-&D-mannosidase was purified to homogeneity from the culture supernatant of Bacillus sp. M-90, which was isolated from soil by enrichment culture on baker's yeast mannan. The purified enzyme had M, 380000 Da, and was comprised of two apparently identical 190000 Da subunits. It had a neutral opt
## Abstract Acidic alpha‐D‐mannosidase purified from the hepatopancreas of __Penaeus monodon__ is pentameric, with a Mr 251,000 as estimated by size‐exclusion FPLC on a Superose^TM^ 12, and retains a hydrophobic domain, being stable to heating at 65°C for 1 h. The specific activity of the purified