Purification and characterization of a hygromycin B phosphotransferase from Streptomyces hygroscopicus
✍ Scribed by Magdalena ZALACAIN; José Manuel PARDO; Antonio JIMÉNEZ
- Book ID
- 115124898
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 374 KB
- Volume
- 162
- Category
- Article
- ISSN
- 1432-1327
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Purification by DNA-cellulose chromatography of acid-soluble DNA-binding proteins (ASB) from Strepromyces hygroscopicus IS described. Two main fractions, ASBII and ASBIII, containing different major protein components were isolated. The dominating protein of ASBIII (19 kd) could be enriched on Srrep
recrystallized from 15 ml of hot absolute ethanol. After standing overnight in refrigerator, 0.85 g of colorless hysoamine, m.p. 183-186", were obtained. [a]g = -k 39.8" (c = 2, H,O). C,H1,N,O, Calc. M + 176.1189 Found M + 176.12056) Preparation of N-Methyl-deoxystreptamine from Destomycin A . This