𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Structure of hygromycin B, an antibiotic from Streptomyces hygroscopicus; The use of CMR. spectra in structure determination, I

✍ Scribed by N. Neuss; K. F. Koch; B. B. Molloy; W. Day; L. L. Huckstep; D. E. Dorman; J. D. Roberts

Publisher
John Wiley and Sons
Year
1970
Tongue
German
Weight
394 KB
Volume
53
Category
Article
ISSN
0018-019X

No coin nor oath required. For personal study only.

✦ Synopsis

recrystallized from 15 ml of hot absolute ethanol. After standing overnight in refrigerator, 0.85 g of colorless hysoamine, m.p. 183-186", were obtained. [a]g = -k 39.8" (c = 2, H,O). C,H1,N,O, Calc. M + 176.1189 Found M + 176.12056) Preparation of N-Methyl-deoxystreptamine from Destomycin A . This amino cyclitol was prepared analogously t o hyosamine; however, starting from 5 g of crude destomycin A, only 210 mg of crystalline, chromatographically impure material were obtained. This was first chroma tographed on Bio-Rad resin (150 g, Dowex, 200400 mesh, OH cycle) using water as eluent yielding 180 mg of still impure amino cyclitol [a12 = -26.6" (H,O) (TLC., Merck Cellulose Plates, MeOH--CHCl,NH,OH 3 : 1 : 2 vol.). This material was rechromatographed on 25 g of cellulose (Schleicher & Schuell, grade 286) using MeOH-CHC13-NH,OH 3 : 1 : 2 vol. solvent mixture. Fractions of 15 ml were collected and examined by TLC. Fractions No. 1-7 were discarded. Fraction No. 8 gave 119 mg of crystalline, chromatographically homogeneous amino cyclitol melting a t 120-126", then resolidifying and melting at 160-162". [a12 = -31,l" Calc. M+ 176.1189 Found 176.11606) Preparation of Destomic Acid from Hygromycin B. This acid was prepared analogously to procedure described for its isolation from destomycin A [3]. The material was identical (IR., NMR., X-ray powder data and TLC.) with an authentic sample from the Japanese investigators4).

M.p. 207-209". [a12 = + 5.3" (c = 2, H,O).

📜 SIMILAR VOLUMES

In Situ Determination Of The Complex Mod
In Situ Determination Of The Complex Modulus From Strain Measurements On An Impacted Structure
✍ B. Lundberg; S. Ödeen 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 205 KB

A method is proposed for in situ determination of the complex modulus of a member of an impact-loaded structure. The modulus was obtained in terms of strains measured at three different cross-sections of a uniform part of the member, by using one-dimensional theory. The potential of the method was e

The rα-structure of partially oriented 1
The rα-structure of partially oriented 1,2,3,5-tetrachlorobenzene determined from its 1H NMR spectra with natural-abundance 13C-satellites. A Demonstration of the Combined Use of Spectra Obtained from Molecules Dissolved in Several Different Liquid Crystals as Solvents for Spin Systems not having enough Dipolar Coupling Constants to Determine the Molecular Structure
✍ G. Dombi; J. Amrein; P. Diehl 📂 Article 📅 1980 🏛 John Wiley and Sons 🌐 English ⚖ 322 KB

## Abstract It is demonstrated that in a case where neither the proton nor the natural‐abundance ^13^C‐satellite spectra of a partially oriented molecule carry enough structural information, one can determine the entire molecular geometry by the combined use of several liquid crystals as solvents.