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Cloning and expression in Escherichia coli of a hygromycin B phosphotransferase gene from Streptomyces hygroscopicus

✍ Scribed by Magdalena ZALACAIN; Francisco MALPARTIDA; Diego PULIDO; Antonio JIMÉNEZ

Book ID
115124897
Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
587 KB
Volume
162
Category
Article
ISSN
1432-1327

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Expression of a neomycin phosphotransferase gene from Streptomyces fradiae in Escherichia coli after interplasmidic recombination
✍ Schupp, Thomas ;Toupet, Christiane ;Stålhammar-Carlemalm, Margaretha ;Meyer, Jür 📂 Article 📅 1983 🏛 Springer 🌐 English ⚖ 928 KB

Plasmid pIJ2 carrying the neomycin phosphotransferase gene of Streptomyces fradiae was fused to E. coli plasmid pBR325 and the hybrid molecules were introduced into E. coli K12 by transformation. The neomycin phosphotransferase gene of the hybrid plasmid was not expressed in E. coli, except after in