New soluble chromogenic substrates were prepared for specific and rapid assays of endo-l,4-/3-xylanases and endo-1 ,4+glucanases. A soluble beechwood 4-0-methyl-D-glucurono-D-xylan was dyed with Remazol brilliant blue R, and hydroxyethylcellulos was coupled to Ostazin brilliant red H-3B. The assays
Purification and characteristics of the endo-1,4-β--glucanase formed by the hybrid fungusTrichoderma harzianum-G-1
✍ Scribed by Zh. T. Tashpulatov; B. G. Baibaev; T. S. Shul'man
- Publisher
- Springer
- Year
- 1996
- Tongue
- English
- Weight
- 187 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0009-3130
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## Abstract __Endo__‐1,3(4)‐β‐glucanase was crystallized from a crude extract of protein solution by addition of a salt A^+^, X^−^. Optical microscopy showed needle‐shaped polydisperse crystals with a mean size of 10 µm. Using transmission electron microscopy with specific settings allows one to ch