A Monte Carlo simulation of the depolymerization of linear homopolymers by endo-enzymes exhibiting random-attack probability and single-attack mechanism: Application to the (1→3),(1→4)-β-D-glucan/endo-(1→3),(1→4)-β-D-glucanase system
✍ Scribed by José V. Carbonell; Luis Izquierdo; José M. Sendra; Paloma Manzanares
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 192 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
A Monte Carlo simulation of the depolymerization of linear homopolymers by specific endo-enzymes exhibiting random-attack probability and a single-attack mechanism has been developed. The program simulates the ''real'' depolymerization versus time of a polydisperse sample of substrate by a specific endo-enzyme. Given the initial mass distribution and concentration of the substrate, the initial concentration of the enzyme, and its Michaelis-Menten constant, the program simulates the evolution of the mass distribution of the substrate with the depolymerization time. When tested against experimental data from the depolymerization of barley (1→ 3),(1→ 4)- -D -glucan by malt endo-(1→3),(1→4)--D-glucanase, monitored using the Calcofluor-FIA method with fluorescent detection, excellent results were obtained.