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Soluble chromogenic substrates for the assay of endo-1,4-β-xylanases and endo-1,4-β-glucanases

✍ Scribed by Peter Biely; Danica Mislovičová; Rudolf Toman

Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
430 KB
Volume
144
Category
Article
ISSN
0003-2697

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✦ Synopsis

New soluble chromogenic substrates were prepared for specific and rapid assays of endo-l,4-/3-xylanases and endo-1 ,4+glucanases. A soluble beechwood 4-0-methyl-D-glucurono-D-xylan was dyed with Remazol brilliant blue R, and hydroxyethylcellulos was coupled to Ostazin brilliant red H-3B. The assays are based on photometric measurements of the enzyme-released dyed fragments soluble in the presence of organic solvents which precipitate the original substrates and their high-molecular-weight fractions. The assays are advantageous for rapid analyses of large amount of samples and also permit evaluation of the activities of both enzymes in the presence of exo+3-glycanases and &giycosidases, at a high level of reducing compounds and viable cells, on the cell surface and on cell membranes and organelles. o 1985 Academic Press, Inc.

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