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Pulsed Field Gradient Pulse Sequence for Heteronuclear [31P–1H] Long Range Correlation

✍ Scribed by Max A. Keniry

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
307 KB
Volume
34
Category
Article
ISSN
0749-1581

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✦ Synopsis

A proton-detected heteronuclear 'H-"P correlation experiment that uses z-pulsed field gradients to select coherences and suppress spectral artefacts is demonstrated for [d(G,T,G,)] 2 , an oligonucleotide which forms a 7 kDa DNA quadruplex in the presence of Na+ and displays considerable spectral overlap. The pulse sequence, when applied to this quadruplex, displays a signal-to-noise ratio comparable to that with the most commonly used proton-detected hereronuclear 1H-31P correlation pulse sequence for macromolecules. Because the heteronuclear couplings are suppressed during the t , delay, linear prediction can be used in the Fl dimension to improve the resolution of the spectrum.

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