Protoplast induction from sporophyte tissues of the heterosporous fernAzolla
โ Scribed by K. Redford; M. D. Berliner; J. E. Gates; R. W. Fisher; B. F. Matthews
- Book ID
- 104617169
- Publisher
- Springer
- Year
- 1987
- Tongue
- English
- Weight
- 1005 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0167-6857
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Protoplasts isolated enzymatically from juvenile leaflets of sporophytes in fern Lyqodium japonicum were inoculated in a MS medium containing NAA 2.7 ฮผm, BAP 2.2 ฮผm, 0.6 M mannitol, and 0.05 M sucrose. Cell division took place within 8 days of culture initiation, and after 30 days cell-clusters with
Factors affecting the regeneration of shoots from Nicotiana plumbaginifolia colonies were studied. As opposed to results obtained with tobacco, phytohormone pretreatments of those colonies did not appreciably affect their subsequent aptitude to develop shoots. Hexoses generally inhibited shoot morph