Nitrogen, carbohydrate and zinc requirements for the efficient induction of shoot morphogenesis from protoplast-derived colonies ofNicotiana plumbaginifolia
✍ Scribed by Michel Caboche
- Publisher
- Springer
- Year
- 1987
- Tongue
- English
- Weight
- 627 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0167-6857
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✦ Synopsis
Factors affecting the regeneration of shoots from Nicotiana plumbaginifolia colonies were studied. As opposed to results obtained with tobacco, phytohormone pretreatments of those colonies did not appreciably affect their subsequent aptitude to develop shoots. Hexoses generally inhibited shoot morphogenesis at concentrations higher than 20 mM, but ribose sustained efficient shoot induction at higher concentration. Shoot induction was promoted by a high nitrogen supply. Nitrate promoted greater shoot induction than ammonium succinate or glutamine. Among the micronutrients tested, zinc was stringently required for efficient shoot induction, concentrations ranging between I 0 and 100 #M being optimal. Under optimized conditions of regeneration, wild type as well as nitrate reductase deficient mutants could be efficiently regenerated.