Division and gametophytic tissue formation from protoplasts of young sporophytes in fernLygodium japonicum

✍ Scribed by M. Maeda; Y. Sugimoto; M. Nakamura; K. Masuda; H. Kaneko; M. Sugai

• Publisher: Springer
• Year: 1990
• Tongue: English
• Weight: 1011 KB
• Volume: 9
• Category: Article
• ISSN: 0721-7714
• DOI: 10.1007/bf00232083

✦ Synopsis

Protoplasts isolated enzymatically from juvenile leaflets of sporophytes in fern Lyqodium japonicum were inoculated in a MS medium containing NAA 2.7 μm, BAP 2.2 μm, 0.6 M mannitol, and 0.05 M sucrose. Cell division took place within 8 days of culture initiation, and after 30 days cell-clusters with 10-15 cells were observed. When the cell-clusters were transferred into fresh hormone-free medium containing no mannitol, they developed rhizoids and protonema-like regenerants. About 2 months later, cordate prothallia developed bearing both antheridia and archegonia.