๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Protoplast culture and plant regeneration inLycium barbarumL.

โœ Scribed by Yakov I. Ratushnyak; Nickolai M. Piven; Vladimir A. Rudas

Publisher
Springer
Year
1989
Tongue
English
Weight
923 KB
Volume
17
Category
Article
ISSN
0167-6857

No coin nor oath required. For personal study only.

โœฆ Synopsis

Al~tract. Protoplasts were isolated from leaves of the woody plant Lycium barbarum L. and cultured in liquid nutrient medium TM-2 at a density of 104-105 cells ml -~ . After ten days of culture, regenerated colonies were transferred to the agar-solidified medium TM-3, and 5-7 days later to regeneration media PRM or TM-4. Formation of shoots was observed after 30-40 days. Completely formed and rooted plants were transferred to the soil. Cytological and morphological analysis of the regenerated plants revealed relative genetic stability of this species in the process 'protoplast --plant'. The results obtained allow us to conclude that L. barbarum can be used in the experiments on somatic hybridization or direct gene transfer.

๐Ÿ“œ SIMILAR VOLUMES

Regeneration ofLycium barbarumL. plants
Regeneration ofLycium barbarumL. plants from leaf tissue, callus culture and callus protoplasts
โœ Yakov I. Ratushnyak; Vladimir A. Rudas; Nickolai M. Piven ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Springer ๐ŸŒ English โš– 996 KB

The possibility of plant regeneration from leaf tissue, callus and callus protoplasts of Lycium barbarum L. has been studied. Leaf segments were cultured on B5 medium (Gamborg et al. 1968) containing 1.5 mg/1 6-benzylaminopurine and 0.5 mg/1 ฮฑ-naphthaleneacetic acid. Regeneration of shoots was initi

Plant regeneration and protoplast cultur
Plant regeneration and protoplast culture ofBrowollia speciosa
โœ J. J. Rybczyล„ski ๐Ÿ“‚ Article ๐Ÿ“… 1989 ๐Ÿ› Springer ๐ŸŒ English โš– 948 KB

Explants from hypcotyls and cotyledons of Browalia speciosa were shown to regenerate plantlets.Protoplasts were isolated from etiolated cotyledon material, and, although callus was readily obtained, plantlet regeneration was not observed using numerous hormone regimes.