Aspects of protoplast culture and plant regeneration
โ Scribed by M. R. Davey; J. B. Power
- Publisher
- Springer
- Year
- 1988
- Tongue
- English
- Weight
- 618 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0167-6857
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Al~tract. Protoplasts were isolated from leaves of the woody plant Lycium barbarum L. and cultured in liquid nutrient medium TM-2 at a density of 104-105 cells ml -~ . After ten days of culture, regenerated colonies were transferred to the agar-solidified medium TM-3, and 5-7 days later to regenerat
Explants from hypcotyls and cotyledons of Browalia speciosa were shown to regenerate plantlets.Protoplasts were isolated from etiolated cotyledon material, and, although callus was readily obtained, plantlet regeneration was not observed using numerous hormone regimes.
Tomato mesophyll protoplasts were cultured in TM2 medium containing 5.7 #M a-naphthaleneacetic acid and 2.4 #M benzyladenine and were incubated either in stationary culture or on an orbital shaker at 25-30 strokes per min, in combination with interval addition of fresh medium. The effects of station