Proton magnetization transfer effect in rat liver lactate

✍ Scribed by Tom Dresselaers; Niki Bergans; Paul Van Hecke; Florent Vanstapel

• Publisher: John Wiley and Sons
• Year: 2002
• Tongue: English
• Weight: 194 KB
• Volume: 47
• Category: Article
• ISSN: 0740-3194
• DOI: 10.1002/mrm.10136

✦ Synopsis

Off-resonance lactate magnetization transfer (MT) experiments were performed on the in situ rat liver under perfused and ischemic conditions. A significant MT effect for lactate methyl protons was observed. The effect was larger for the ischemic condition than for the perfused condition, and was largest in the blood-filled ischemic livers. The size of the motionally restricted lactate pool, determined using a two-pool model fit, was estimated to be about 1% in perfused livers and about 1.8-2.5% after more than 1 hr of onset of ischemia, suggesting that lactate in liver is almost fully NMR-visible. The MT data for both the perfused and the ischemic condition appeared to be better approximated when assuming a superLorentzian lineshape for the immobile pool rather than a Gaussian lineshape. Finally, the experiments demonstrated a coupling between the lactate methyl and water protons, which may be mediated by macromolecules.