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Proto-oncogene amplification and homogeneously staining regions in human breast carcinomas

✍ Scribed by Claude Saint-Ruf; Michéle Gerbault-Seureau; Evani Viegas-Péquignot; Brigitte Zafrani; Roland Cassingena; Dr. Bernard Dutrillaux

Book ID
102844478
Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
741 KB
Volume
2
Category
Article
ISSN
1045-2257

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✦ Synopsis

Cytogenetic studies on fresh human breast cancers revealed that homogeneously staining regions (HSRs), which are assumed t o represent DNA amplification, are observed in almost half of the cases. To search for a possible relationship between HSRs and proto-oncogene amplification, I 6 proto-oncogenes, including ERBB2, were studied by Southern blot analysis in four tumors with two or three HSRs, and in three tumors without HSRs. Only four proto-oncogenes were found t o be amplified in at least one tumor each HST and lNT2 ( X 3), MYC (X 2-3), and FES ( X > 10). The large sizes of the HSRs, which each corresponded to several percent of the haploid genome, were hardly compatible with the low rate of amplification, except for FESand then only if a large adjacent segment was co-amplified. This incomplete correlation was demonstrated by in situ hybridization, using biotinylated probes, which showed fluorescent spots on only one HSR for FES in one tumor and for lNT2 in another one. Our results indicate that most of the large amplifications corresponding t o HSRs do not involve the proto-oncogenes usually studied in breast cancer. The large amplification of FES, detected in one tumor, may be coincidental.

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