Identification of amplified DNA sequences in breast cancer and their organization within homogeneously staining regions

✍ Scribed by Martine Muleris; Anna Almeida; Michèle Gerbault-Seureau; Bernard Malfoy; Bernard Dutrillaux

• Book ID: 102843395
• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 666 KB
• Volume: 14
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/gcc.2870140302

✦ Synopsis

A modified comparative genomic hybridization (mCGH) technique was used t o identify and map amplified DNA sequences in six homogeneously staining regions (hsr) from three primary breast carcinomas. Five different chromosomal regions and bands were identified as sites of amplification: 8p I, I7q2 I. I, I7q23 (two cases), 19q I 3.3, and 20q I 3.3. The mCGH site located on I7q2 I. I was demonstrated to correspond to a 50-1 00-fold amplification of ERBBZ. Further in situ hybridization experiments were used t o confirm the mCGH results and t o characterize the organization of the amplified sequences within the hsr. In five of six instances, two or more chromosomal regions were found amplified in the same hsr. In the tumor with the less modified karyotype, the two hsr comprised DNA sequences from three different chromosomes and showed different patterns of amplification. In the tumor with the most rearranged karyotype, the hsr-carrying chromosomes were formed by the translocation and amplification of sequences from three or four different chromosomal sites. This illustrates the complexity of the amplification process in breast cancers. Genes Chromosom Cancer /4:155-163 (1995).