Proteomics in Functional Genomics: Protein Structure Analysis

✍ Scribed by Ettore Appella, David Arnott, Kazuyasu Sakaguchi (auth.), Prof. Dr. P. Jollès, Prof. Dr. H. Jörnvall (eds.)

• Publisher: Birkhäuser Basel
• Year: 2000
• Tongue: English
• Leaves: 239
• Series: EXS 88
• Edition: 1
• Category: Library

✦ Synopsis

A wealth of information has accumulated over the last few years on the human genome. The new insights have completely changed the focus of protein analysis. It is no longer time-consuming analysis of unknown products, but rather selective identifications of individual forms, modifications and processings, and overall analysis of global protein outputs from cells and tissues in health and disease. This book gears to the rising need of sensitive, accurate, and fast separation and identification techniques in proteomics. It discusses current methodologies of modern protein analysis, from isolation and sample preparation, over analysis and identification, to final characterization. Several evaluations concentrate on the now productive approaches of two-dimensional gel electrophoresis and mass spectrometry, but alternative methods and further perspectives are also outlined. The book includes an overlook over current databases to connect protein analysis data with all available information,...

✦ Table of Contents

Front Matter....Pages I-XIII
Proteome mapping by two-dimensional polyacrylamide gel electrophoresis in combination with mass spectrometric protein sequence analysis....Pages 1-27
Techniques for sample preparation including methods for concentrating peptide samples....Pages 29-42
Sample preparation by SDS/PAGE and in-gel digestion....Pages 43-54
High-resolution two-dimensional gel electrophoresis and protein identification using western blotting and ECL detection....Pages 55-67
Nanospray mass spectrometry in protein and peptide chemistry....Pages 69-79
MALDI-TOF mass spectrometry in protein chemistry....Pages 81-97
The chemistry of protein sequence analysis....Pages 99-117
The alkylated thiohydantoin method for C-terminal sequence analysis....Pages 119-131
Ladder sequencing....Pages 133-144
New approaches for innovations in sensitive Edman sequence analysis by design of a wafer-based chip sequencer....Pages 145-157
Analysis of glycoprotein-derived glycopeptides....Pages 159-186
Lipopeptide preparation and analysis....Pages 187-198
Phosphopeptide analysis....Pages 199-213
Bioinformatics in protein analysis....Pages 215-231
Back Matter....Pages 233-236

✦ Subjects

Life Sciences, general; Biomedicine general